Perfusion

Labolatory of Liver Research(2008)

Perfusion: Primary Liver Cell Isolation Team

 

 

  • Under anesthesia, perfuse liver. Collected liver cells were centrifuged, and we could isolate primary Hepatocytes, Hepatic Stellate Cells (HSCs), Kupffer Cells (KCs), Liver Sinusoidal Endothelial Cells (LSECs) with concentration gradient solutions.
  • Isolated cells were used for in vitro experiment, and we could observe the effect of diverse diet, medicine or chemicals on each cells.
  • More purified cells could be sorted with FACS (Fluorescence Activated Cell Sorting) or MACS(Magnetic-activated Cell Sorting) technologies with specific cell surface markers.
Hepatocytes

Hepatic Stellate Cells

Kupffer Cells

Exosome Uptake in Hepatic Stellate Cells

Phase contrast

Auto-fluorescence (Vit A)

DiI-stained exosomes (16hr)

Merge (D2 HSC)